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Detection of metabolites of toxic alkylmethylphosphonates in biological samples
Author(s) -
Shih M. L.,
Smith J. R.,
McMonagle J. D.,
Dolzine T. W.,
Gresham V. C.
Publication year - 1991
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200201111
Subject(s) - derivatization , chemistry , chromatography , mass spectrometry , gas chromatography , chemical ionization , sarin , gas chromatography–mass spectrometry , biological materials , urine , biological fluids , mass spectrum , environmental chemistry , ionization , organic chemistry , biochemistry , biology , enzyme , ion , biological system , acetylcholinesterase
The major metabolites and breakdown products of some toxic organophosphonates are their respective alky‐methylphosphonic acids. These acids ionize at physiological pH and are not amenable to gas chromatographic analysis in their underivatized forms. Their detection in biological samples has been difficult because of their presence at only trace levels. Existing analytical methods were developed mainly for measuring these phosphonic acids in environmental samples and at higher concentrations. In this study, we devised a gas chromatographic/mass spectrometric method to provide confirmation and quantification of the organophosphonic acids of soman (GD), sarin (GB) and GF in blood and urine. This report describes the various derivatization conditions that we have studied and demonstrates the characteristic mass spectra by different ionization techniques.