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Fast atom bombardment tandem mass spectrometric analysis of N ‐carbamoylamino acids
Author(s) -
Inoue Yoshito,
Ohkura Takashi,
Matsumoto Isamu,
Rudewicz Patrick J.
Publication year - 1991
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200201004
Subject(s) - chemistry , fast atom bombardment , protonation , tandem mass spectrometry , moiety , collision induced dissociation , mass spectrometry , dissociation (chemistry) , molecule , tandem , mass spectrum , ion , nitrogen atom , polyatomic ion , chromatography , stereochemistry , organic chemistry , materials science , composite material , group (periodic table)
Using fast atom bombardment (FAB) and tandem mass spectrometry (MS/MS), we examined 12 synthetic N ‐carbamoylamino acids (CAA) as tert ‐butyldimethylsilyl (TBDMS) derivatives. In FAB mass spectrometry and FAB MS/MS, spectra of protonated molecules for CAA provide specific cleavages involving the TBDMS carbamoyl moiety. The daughter scan spectrum of the parent ion indicated that it was useful for structural elucidation and differentiation of structural isomers of CAA. We have also identified each CAA separately in a mixture using a neutral loss scan for characteristic ions. In addition, we demonstrated that CAA in urine samples from patients with ornithine carbamoyl transferase deficiency gave collision‐induced dissociation (CID) spectra which correspond well with CID spectra obtained using synthetically prepared CAA.