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Plasma desorption mass spectrometry of haemoglobin tryptic peptides for the characterization of a Hungarian α‐chain variant
Author(s) -
Jensen O. Nørregaard,
Roepstorff P.,
Rozynov B.,
Horanyi M.,
Szelenyi J.,
Hollan S. R.,
Aseeva E. A.,
Spivak V. A.
Publication year - 1991
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200201002
Subject(s) - mass spectrometry , chemistry , chromatography , characterization (materials science) , protein mass spectrometry , desorption , tandem mass spectrometry , materials science , organic chemistry , nanotechnology , adsorption
S ‐Aminoethylated‐α A and ‐β A globin tryptic peptides separated by reversed‐phase high‐performance liquid chromatography have been analysed by plasma desorption mass spectrometry. Almost all the expected α A and β A tryptic fragments were tentatively assigned relative to the known globin chain sequences based on the molecular weight obtained by plasma desorption mass spectrometric analysis of the purified peptides. The application of plasma desorption mass spectrometry for structure elucidation of a haemoglobin α‐chain variant revealed the first case of Hb Hasharon in Hungary.