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Deuterium‐labelling and quantitative measurement of Ketotifen in human plasma by gas chromatography/negative ion chemical ionization mass spectrometry
Author(s) -
Leis Hans Jörg,
Malle Ernst
Publication year - 1991
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200200806
Subject(s) - chemistry , mass spectrometry , chromatography , chemical ionization , isotope dilution , deuterium , gas chromatography , ionization , ion , analytical chemistry (journal) , organic chemistry , physics , quantum mechanics
A gas chromatographic/mass spectrometric assay for the anti‐anaphylactic agent Ketotifen is described. The method is based on negative ion chemical ionization, utilizing the electrophoric nature of the underivatized drug. A simple procedure for deuteration of the intact target molecule is presented, thus enhancing specificity by internal standardization via stable isotope dilution. The assay was applied to pharmacokinetics of the drug in man.