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Incorporation of tandem mass spectrometric detection to the analysis of peptide mixtures by continuous flow fast atom bombardment mass spectrometry
Author(s) -
Ackermann Bradley L.,
Coutant John E.,
Chen TengMan
Publication year - 1991
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200200707
Subject(s) - chemistry , chromatography , mass spectrometry , tandem mass spectrometry , fast atom bombardment , analytical chemistry (journal) , peptide , protein mass spectrometry , biochemistry
The ability to acquire structurally informative daughter ion spectra for individual peptides undergoing separation and analysis by continuous flow fast atom bombardment (CF FAB) is demonstrated. To illustrate the potential of this methodology, tryptic and chymotryptic digests of the 29‐residue peptide glucagon were analyzed by CF FAB using mass specrromctric and tandem mass spectrometric detection in consecutive analyses. Daughter ion spectra were recorded using B / E linked scans for the major hydrolysis products observed by liquid chromatography/mass spectrometry. The peptide mixtures were separated by gradient capillary high‐performance liquid chromatography with the FAB matrix being added post‐column using a coaxial flow interface between the column and flow probe. The entire effluent (3 μl min −1 ) was sampled by the mass spectrometer. Results obtained using less than 300 pmol of digested glucagon indicated several advantages to tandem mass spectrometric detection including the ability to confirm identities for products of enzymatic digestion and the potential use of this method for tandem sequence analysis of peptide mixtures.