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A moving belt device to couple high‐performance liquid chromatography and chemical reaction interface mass spectrometry
Author(s) -
Moini Mehdi,
Abramson Fred P.
Publication year - 1991
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200200512
Subject(s) - thermospray , mass spectrometry , chemistry , vaporizer , high performance liquid chromatography , chromatography , analytical chemistry (journal) , gas chromatography , molecule , tandem mass spectrometry , organic chemistry , selected reaction monitoring , medicine , pathology
A device is described to enable selective detection of 13 C‐ and 15 N‐labeled compounds following separation by high‐performance liquid chromatography (HPLC). A thermospray vaporizer deposits the materials eluting from the HPLC column onto a continuously moving endless belt. The belt carries these compounds into the chemical reaction interface, where a microwave‐induced helium plasma converts complex organic molecules in the presence of a reactant gas into small stable molecules that are detected by the mass spectrometer. Chromatograms showing only compounds enriched with 13 C and 15 N can be obtained by subtracting the abundance of naturally occurring isotopes from the observed M + 1 signal. This work demonstrates the feasibility of this approach and encourages its further development.