Premium
Identification of intact glucosinolates using direct coupling of high‐performance liquid chromatography with continuous‐flow frit fast atom bombardment tandem mass spectrometry
Author(s) -
Kokkonen P. S.,
van der Greef J.,
Niessen W. M. A.,
Tjaden U. R.,
ten Hove G. J.,
van de Werken G.
Publication year - 1991
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200200505
Subject(s) - chemistry , chromatography , fast atom bombardment , tandem mass spectrometry , tandem , mass spectrometry , analytical chemistry (journal) , ion , frit , organic chemistry , materials science , metallurgy , composite material
Intact glucosinolates have been separated and identified in standard mixtures using liquid chromatography (LC) and continuous‐flow frit fast atom bombardment tandem mass spectrometry. The separation of individual glucosinolates is achieved by using a conventional‐bore LC column with an aqueous ammonium acetate‐acetonitrile gradient. The mandatory flow rate reduction is achieved by using a pneumatic splitter. The daughter ion spectra after collision‐induced dissociation of the characteristic [M − H] − anion of the glucosinolates yields extensive structure information. Both group‐ and compound‐specific fragments and group‐characteristic neutral losses are observed. Strategies for identification of unknown glucosinolates in a mixture from their negative ion tandem mass spectra are indicated.