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Reaction mechanism and fragment ion structure determination of deprotonated small oligosaccharides, studied by negative ion fast atom bombardment (tandem) mass spectrometry
Author(s) -
Dallinga Jan W.,
Heerma Wigger
Publication year - 1991
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200200410
Subject(s) - chemistry , fast atom bombardment , fragmentation (computing) , mass spectrometry , tandem mass spectrometry , ion , molecule , analytical chemistry (journal) , deuterium , mass spectrum , polyatomic ion , top down proteomics , selected reaction monitoring , chromatography , organic chemistry , atomic physics , physics , computer science , operating system
The negative ion mass spectrometric characteristics of a series of di‐ and trisaccharides and the tetrasaccharide stachyose have been studied using fast atom bombardment mass spectrometry. The molecular weight of the compounds can easily be derived from their mass spectra, which all show an abundant [M − H] − ion peak. The application of metastable ion and collisional activation techniques to selected pseudomolecular and fragment ions appears to be appropriate for the determination of the position and anomeric type of linkage in the molecules, and provides information concerning the monosaccharide units involved. Important fragmentation reactions have been traced and reaction mechanisms, supported by deuterium labelling experiments, are proposed. An experiment describing the application of the findings of this study to a glycosphingolipid molecule demonstrates its potential value for biological systems.