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Collisionally activated dissociation spectra of sodiated peptides and peptide amides
Author(s) -
Tomer Kenneth B.,
Deterding Leesa J.,
Guenat Christian
Publication year - 1991
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200200305
Subject(s) - chemistry , ion , dissociation (chemistry) , peptide , deuterium , spectral line , mass spectrum , crystallography , analytical chemistry (journal) , chromatography , organic chemistry , biochemistry , physics , quantum mechanics , astronomy
The CAD spectra of the [M + Na] + ions of a series of peptide free acids and amides have been acquired. The spectra of the sodiated amides were found to differ significantly from those of the sodiated free acids due to absence of the [B n + Na + OH] + ions in the spectra of the amides. The abundances of [Y n + Na − H] + ions, [W n + Na] + ions and [D n + Na] + ions is increased in the spectra of the sodiated peptides while internal fragments are reduced in abundance. Deuterium labeling shows that the hydrogens lost in the formation of [Y n + Na − H] + ions and the [A n + Na − H] + ions are non‐exchangeable.