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Application of FRIT fast atom bombardment liquid chromatography/mass spectrometry for the determination of acetylcholine levels in rat brain regions
Author(s) -
Ikarashi Yasushi,
Itoh Kouichi,
Maruyama Yuji
Publication year - 1991
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200200106
Subject(s) - chromatography , chemistry , mass spectrometry , fast atom bombardment , frit , derivatization , high performance liquid chromatography , acetylcholine , gas chromatography , deuterium , analytical chemistry (journal) , materials science , medicine , physics , quantum mechanics , metallurgy , endocrinology
This report describes the use of FRIT fast atom bombardment (FAB) liquid chromatography/mass spectrometry for the analysis of acetylcholine in rat brain regions. Direct assessment of acetylcholine levels is possible without the need for either derivatization or extensive sample preparation. Quantification is accomplished by monitoring intact molecular cations of acetylcholine and a deuterated internal standard. The results are compared with those obtained by conventional pyrolysis gas chromatography/mass spectrometry and by liquid chromatography with electrochemical detection.