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Low‐energy collision‐induced dissociation of protonated polyamino alcohol derivatives of peptides and N‐terminal blocked peptides
Author(s) -
Fujioka Mamoru,
Koda Shigetaka,
Yasuda Tsutomu
Publication year - 1990
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200191304
Subject(s) - dissociation (chemistry) , protonation , cleavage (geology) , chemistry , peptide , ion , molecule , sequence (biology) , alcohol , stereochemistry , peptide sequence , collision induced dissociation , organic chemistry , chromatography , mass spectrometry , biochemistry , materials science , tandem mass spectrometry , fracture (geology) , composite material , gene
This investigation reports the low‐energy collision‐induced dissociation of the protonated molecules of polyamino alcohols, formed by chemical reduction of synthetic peptides and N‐terminal blocked peptides, in order to evaluate its potential for peptide sequence determination. The CH 2 NH cleavage with charge retention on the Nterminus was prominent, and the entire sequence of ions produced in this manner was observed. Some of the sequence ions arising from CH 2 NH cleavage accompanied by migration of two hydrogens, and NHC α H cleavage with charge retention on the C‐terminus, also occurred prominently. In addition, a great number of internal fragment ions were produced in relatively high abundance; these provided supplementary sequence information and were, in some cases, critical in determining the complete sequence. The usefulness of this method was exemplified by its application to the sequence determination of bacterial lipopeptides.