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Structural analysis of oligosaccharides by tandem mass spectrometry: Collisional activation of sodium adduct ions
Author(s) -
Orlando Ron,
Allen Bush C.,
Fenselau Catherine
Publication year - 1990
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200191202
Subject(s) - chemistry , adduct , oxonium ion , glycosidic bond , mass spectrometry , molecule , ion , protonation , mass spectrum , tandem mass spectrometry , fragmentation (computing) , dissociation (chemistry) , organic chemistry , chromatography , computer science , enzyme , operating system
The collisionally activated dissociation (CAD) spectra of the sodium adduct ions of several oligosaccharides were obtained and compared to CAD spectra of the protonated molecules. Isomeric lacto‐ N ‐fucopentose I and lacto‐ N ‐fucopentose II, stachyose and maltodecaose are discussed here. Both natriated and protonated molecules provide familiar oxonium ion series through bond scissions in the glycosidic linkages under 8 keV collisions with helium. Natriated adducts also undergo two‐bond cross‐ring cleavages analogous to those observed in the laser desorption mass spectra of oligosaccharides. The relative contributions of glycosidic and cross‐ring ion series in the CAD spectra of sodium adducts of carbohydrates are dependent on and can be controlled by collision energies in the 1‐8 keV range.