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Liquid chromatography/mass spectrometry of plasma glucose and secreted glucuronate for metabolic studies in humans
Author(s) -
Reid Steve,
Shackleton Cedric,
Wu Ken,
Kaempfer Suzanne,
Hellerstein Marc K.
Publication year - 1990
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200190903
Subject(s) - glucuronate , mass spectrometry , chromatography , chemistry , liquid chromatography–mass spectrometry , biochemistry
Negative ion thermospray liquid chromatographic/mass spectrometric methods have been developed for the determination of isotopic enrichments for glucose and acetaminophen—uridine diphosphate glucuronate from (1‐ 2 H 1 )glucose, (1‐ 2 H 1 )galactose and (2‐ 13 C)acetate in humans. The error of estimate ranged from below 1% to 5%. The advantages of the method include fast analysis (up to 35 per hour), eased sample preparation, good precision, sensitivity comparable with gas chromatography/mass spectrometry and better than with isotope ratio mass spectrometry.