Premium
An improved chemical ionization assay for mevalonic acid
Author(s) -
Hoffmann Georg F.,
Sweetman Lawrence
Publication year - 1990
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200190809
Subject(s) - mevalonic acid , chromatography , chemistry , chemical ionization , gas chromatography , mass spectrometry , ionization , biochemistry , ion , organic chemistry , enzyme , reductase
The determination of mevalonic acid by gas chromatography/mass spectrometry (GC/MS) is complicated by poor extractability and multiple product formation. Reliable quantification down to physiological levels was achieved by isolating mevalonic acid by liquid partition chromatography and quantifying the underivatized lactone by means of ammonia chemical ionization, selected ion monitoring, capillary GC/MS. ( 2 H 3 )mevalonic acid served as the internal standard. The method appears to be suitable for the study of mevalonic aciduria as well as the evaluation of different therapeutic regimens in patients with hypercholesterolaemia.