Gas chromatography/negative ion chemical ionization/tandem mass spectrometric quantification of indomethacin in plasma and synovial fluid | Zendy

Dawson Michael | Zendy; Smith Michael D. | Zendy; McGee C. M. | Zendy

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Gas chromatography/negative ion chemical ionization/tandem mass spectrometric quantification of indomethacin in plasma and synovial fluid

Author(s) -

Dawson Michael,

Smith Michael D.,

McGee C. M.

Publication year - 1990

Publication title -

biomedical and environmental mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 121

eISSN - 1096-9888

pISSN - 0887-6134

DOI - 10.1002/bms.1200190802

Subject(s) - chromatography , chemistry , synovial fluid , chemical ionization , tandem , ion , plasma , ionization , analytical chemistry (journal) , materials science , organic chemistry , medicine , pathology , alternative medicine , osteoarthritis , physics , quantum mechanics , composite material

A procedure for the quantification of sub‐nanogram per millilitre concentrations of indomethacin in plasma and synovial fluid has been developed using gas chromatography/negative ion chemical ionization/tandem mass spectrometry. The procedure is based on selected ion monitoring of the daughter ion at m / z 312 generated following decarboxylation of the 35 Cl isotope peak of the indomethacin anion ( m / z 356) formed from the pentafluorobenzyl ester of indomethacin in the ion source of the mass spectrometer. The internal standard, d 2‐indomethacin, has a daughter ion at m / z 316 formed from the corresponding 37 Cl isotope peak. The limit of quantification was 0.1 ng ml −1 for both plasma and synovial fluid.

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