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Structural analysis of macrotetralide antibiotic mixtures using collision‐induced dissociation mass spectrometry
Author(s) -
Vincenti Marco,
Guglielmetti Gianfranco,
Andriollo Nunzio,
Cassani Giorgio
Publication year - 1990
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200190407
Subject(s) - dissociation (chemistry) , monomer , fragmentation (computing) , collision induced dissociation , protonation , chemistry , mass spectrometry , ion , cleavage (geology) , mass spectrum , molecule , tandem mass spectrometry , computational chemistry , chromatography , organic chemistry , materials science , polymer , fracture (geology) , computer science , operating system , composite material
Collision‐induced dissociation of protonated molecular ions in a mixture of macrotetralide antibiotics has been used to determine the sample make‐up, as well as the structures of the components in the mixture. Upon 3 keV gaseous collisional activation the near‐tetrameric structure of the macrotetralides is opened and can then fragment further to give oligomeric ions through consecutive losses of monomeric units. The fragment ions generated indicate which monomers are present and also their sequence in the cyclic assembly. The fragmentation mechanism involves cleavage of an ester bond to give an undissociated carboxyl group. This cleavage may take place remote from the charge site.