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Determination of tebufelone, a new anti‐inflammatory drug, in plasma and tissue using capillary gas chromatography/stable isotope dilution mass spectrometry
Author(s) -
Eichhold Thomas H.,
Doyle Matthew J.
Publication year - 1990
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200190405
Subject(s) - chromatography , chemistry , detection limit , mass spectrometry , isotope dilution , gas chromatography , capillary action , coefficient of variation , analytical chemistry (journal) , selected ion monitoring , gas chromatography–mass spectrometry , extraction (chemistry) , materials science , composite material
A capillary gas chromatographic/mass spectrometric method for the determination of tebufelone, a new anti‐inflammatory drug, in plasma and rat paw tissue is described. Trideuteriated tubufelone was employed as an internal standard and the drug quantified by selected ion monitoring using a mass‐selective detector following positive electron ionization (70 eV). Tebufelone was recovered from plasma (>93%) and pulverized paw tissue (>88%) by ethereal extraction. Standard curves were linear between 5 ng ml −1 and 5 μg ml −1 with a detection limit (S/N = 2) of 2 ng ml −1 . The coefficient of variation for successive injections of standards (200 ng ml −1 ) or samples (10–1500 ng ml −1 ) was 7.3% and 8.5%, respectively.