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Positive and negative ion 252 Cf plasma desorption mass spectrometry of polar agrochemical metabolites
Author(s) -
Allmaier Guenter,
Schmid Erich R.,
Roepstoff Peter
Publication year - 1990
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200190205
Subject(s) - chemistry , chromatography , mass spectrometry , desorption , mass spectrum , adsorption , nitrocellulose , organic chemistry , biochemistry , membrane
Abstract The use of 252 Cf plasma desorption (PD) mass spectrometry for the identification of highly polar agrochemical metabolites is illustrated with metabolites of azinphos‐methyl, ‐ethyl, demeton‐ S ‐methyl and demeton‐ S ‐methyl sulphoxide. Three different classes of metabolites were used, namely the monosodium salt of organophosphorus acid ester, the glycoside and the sodium salt of sulphonic acid. Positive and negative ion mass spectra were recorded for different sample preparation techniques. The electrospray method and nitrocellulose adsorption with sample application by spin‐drying with and without a rinsing step was applied and evaluated. PD mass spectrometry has proved to be useful in providing fast information on the molecular weight of all three classes of metabolites, but the method has failed to give fragment ions. The best result in terms of sensitivity and low background were obtained using the nitrocellulose spin‐drying method without a washing step. It has been found to be a very valuable technique for the rapid identification of non‐volatile or thermally labile agrochemical metabolites after high‐performance liquid chromatographic isolation from plants.