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Gas chromatography/negative chemical ionization mass spectrometry of intact glucuronides
Author(s) -
Brown Sharon Y.,
Garland William A.,
Fukuda Elaine K.
Publication year - 1990
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200190105
Subject(s) - chemistry , chemical ionization , mass spectrometry , chromatography , gas chromatography , gas chromatography–mass spectrometry , bromide , fast atom bombardment , direct electron ionization liquid chromatography–mass spectrometry interface , glucuronide , ionization , ion , organic chemistry , metabolite , biochemistry
Gas chromatography‐negative chemical ionization mass spectrometry (GC–NCIMS) has been applied to the analysis of O ‐ and S ‐glucuronides. The glucuronides were derivatized with pentafluorobenzyl (PFB) bromide and Tri‐Sil, and analyzed by GC–NCIMS. All glucuronides gave a large [M—PFB] − ion indicative of the molecular weight of the compound. This methodology has been successfully applied to the identification of a glucuronide of unknown structure.