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Measurement of ethyl carbamate in blood by capillary gas chromatography/mass spectrometry using selected ion monitoring
Author(s) -
Hurst Harrell E.,
Kemper Raymond A.,
Kurata Norimitsu
Publication year - 1990
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200190104
Subject(s) - ethyl carbamate , carbamate , chromatography , chemistry , mass spectrometry , selected ion monitoring , extraction (chemistry) , gas chromatography–mass spectrometry , detection limit , gas chromatography , organic chemistry , food science , wine
Methodology is presented for convenient, reproducible and direct measurement of blood concentrations of ethyl carbamate, an experimental animal carcinogen. Extraction techniques requiring 20 μl of blood and selected ion monitoring using ethyl ( 13 C, 15 N)carbamate as internal standard enabled quantification of ethyl carbamate concentrations ranging from 50 ng ml −1 to 100 m̈g ml −1 . Coefficients of variation at several representative concentrations averaged less than 4%. The method was used to determine the time course of elimination of ethyl carbamate from mice receiving doses of 125 m̈mol kg −1 .