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Molecular species analysis of phospholipids by negative ion fast atom bombardment mass spectrometry: Application of surface precipitation technique
Author(s) -
Chen Su,
Benfenati Emilio,
Fanelli Robert,
Kirschner Günther,
Pregnolato Francesco
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200181204
Subject(s) - fast atom bombardment , mass spectrometry , chemistry , precipitation , ion , chromatography , molecule , secondary ion mass spectrometry , polar , analytical chemistry (journal) , organic chemistry , physics , astronomy , meteorology
Eight phospholipidic classes from bovine brain cortex and soybean were prepared and purified by preparative high‐performance and liquid chromatography, and their molecular species were identified by negative ion fast atom bombardment mass spectrometry using the ‘surface precipitation’ method. Its main advantages are: (i) clear‐cut and abundant diagnostic ions for structural elucidation of the species; (ii) fragments characteristic of the main fatty acids, the polar head‐group and the molecule frame in phospholipids; (iii) less background caused by the liquid matrix.