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Quantitative determination of arecoline in plasma by gas chromatography chemical ionization mass spectrometry
Author(s) -
Hayes M. J.,
Khemani L.,
Bax M.,
Alkalay D.
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200181109
Subject(s) - chemistry , chromatography , mass spectrometry , arecoline , gas chromatography , analyte , chemical ionization , gas chromatography–mass spectrometry , selected ion monitoring , extraction (chemistry) , direct electron ionization liquid chromatography–mass spectrometry interface , analytical chemistry (journal) , ethyl acetate , ionization , ion , organic chemistry , biochemistry , receptor , muscarinic acetylcholine receptor
A capillary gas chromatography/mass spectrometry (GC/MS) method for the quantitative analysis of arecoline in plasma has been developed for concentrations in the range 1–50 ng ml −1 . Hexadeuterated arecoline was utilized as the internal standard. The removal of drug from plasma was accomplished by a two‐step liquid/liquid extraction procedure involving a wash step followed by extraction with 5% triethyl amine in ethyl acetate. The GC/MS determinations were carried out with temperature‐programmed capillary GC and ammonia chemical ionization mass spectrometry. The [M + H] + ions of both analyte and internal standard were monitored at m/z 156 and 162, respectively. The method is linear and has sufficient sensitivity, precision, accuracy and selectivity for analysis of drug levels in human plasma.