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Fragmentation of deuteromethanol from 3,3′,4,4′‐[ 2 H 4 ]‐prostanoid pentafluorobenzylester/methoxime/trimethylsilylether derivatives by collisionally activated decomposition
Author(s) -
Mackert Gerhard,
Seyberth Hannsjörg W.,
Schweer Horst
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200181015
Subject(s) - chemistry , fragmentation (computing) , deuterium , prostanoid , isotope dilution , methanol , ion , mass spectrometry , mass spectrum , analytical chemistry (journal) , chemical ionization , ionization , chromatography , organic chemistry , biochemistry , physics , receptor , quantum mechanics , computer science , operating system
In negative ion chemical ionization mass spectra of prostanoid pentafluorobenzylester(PFB)/methoxime(MO)/ trimethylsilylether(TMS) derivatives [M‐PFB] − is the most abundant fragment ion. Collisionally activated decomposition (CAD) spectra of this ions show nearly only fragmentation of trimethylsilanol (TMSOH), (CH 3 ) 2 SiCH 2 , carbon dioxide and methanol. CAD spectra of [M‐PFB] − ions of 3,3′,4,4′‐deuterated PGE 2 and 6‐oxo‐PGF 1α PFB/MO/TMS derivatives fragmentation of methanol and deuteromethanol (CH 3 OD) is observed. The ratio of CH 3 OH/CH 3 OD is about 3:1 (PGE 2 ) and 9:1 (6‐oxo‐PGF 1α ) respectively. This loss of deuterium in the internal standard needs to be considered when employing the isotope dilution technique in quantitative prostanoid analysis and tandem mass spectrometry.