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Fast atom bombardment mass spectrometry and selective acid hydrolysis for the analysis of partially modified retro‐inverso peptide analogues
Author(s) -
De Angelis Francesco,
Ceccarelli Stefano,
Viscomi Giuseppe C.,
Pinori Massimo,
Verdini Antonio S.
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200181005
Subject(s) - fast atom bombardment , chemistry , mass spectrometry , protonation , peptide , fragmentation (computing) , hydrolysis , mass spectrum , ion , acid hydrolysis , chromatography , stereochemistry , organic chemistry , biochemistry , computer science , operating system
Fast atom bombardment (FAB) mass spectrometry has been successfully applied to the analysis of partially modified retro‐inverso peptide isomers. The spectra are characterized by abundant protonated molecular ions and also by sequence ions due to fragmentation of the inverted bonds. Unambiguous information, as to the nature and the position in the backbone of the amino acids involved in the partial modification of the structure, are given by using a combination of FAB mass spectrometry and partial, selective acid hydrolysis, without separation of the resulting peptide mixtures.