Premium
The analysis and characterization of isomeric metabolites of temelastine by the combined use of thermospray liquid chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry
Author(s) -
Blake Timothy J. A.,
Beattie Iain G.
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200181004
Subject(s) - thermospray , chromatography , chemistry , mass spectrometry , liquid chromatography–mass spectrometry , tandem mass spectrometry , glucuronidation , high performance liquid chromatography , glucuronide , metabolite , selected reaction monitoring , organic chemistry , biochemistry , enzyme , microsome
This paper describes the development and application of a combined thermospray liquid chromatographic/mass spectrometric and liquid chromatographic/tandem mass spectrometric method for distinguishing between five isomeric metabolites of Temelastine, comprising four hydroxylated metabolites and one N ‐oxide. The method allows the unambiguous characterization of all of the isomers either on their own or in the presence of each other. Clear results were obtained for the characterization of these metabolites in biological samples. Temelastine showed extensive phase I and phase II metabolism and the methodology was used to study the aglycone structures of the glucuronide conjugates derived from the hydroxylated metabolites. Photo‐diode array ultraviolet spectroscopy was used as a complementary technique to help elucidate the site of glucuronidation in these species.