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Ultraviolet–laser desorption/ionization mass spectrometry of femtomolar amounts of large proteins
Author(s) -
Karas M.,
Ingendoh A.,
Bahr U.,
Hillenkamp F.
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200180931
Subject(s) - mass spectrometry , ultraviolet , atmospheric pressure laser ionization , ionization , chemistry , laser , desorption , surface enhanced laser desorption/ionization , chromatography , analytical chemistry (journal) , sample preparation in mass spectrometry , materials science , ion , optoelectronics , electrospray ionization , electron ionization , optics , physics , adsorption , organic chemistry
Proteins with molecular weights between 14 000 and 175 000 daltons were investigated by matrix‐assisted laser desorption/ionization mass spectrometry. High abundance molecular ions were obtained with absolute sample amounts ranging from 5 pmol down to 50 fmol, allowing molecular weight determination with an accuracy between 0.1 and 0.2%. The amount of sample needed for an analysis is determined by the preparation rather than by the mass analysis step; the majority of the sample can be regained after analysis.