Ultraviolet–laser desorption/ionization mass spectrometry of femtomolar amounts of large proteins | Zendy

Karas M. | Zendy; Ingendoh A. | Zendy; Bahr U. | Zendy; Hillenkamp F. | Zendy

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Ultraviolet–laser desorption/ionization mass spectrometry of femtomolar amounts of large proteins

Author(s) -

Karas M.,

Ingendoh A.,

Bahr U.,

Hillenkamp F.

Publication year - 1989

Publication title -

biomedical and environmental mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 121

eISSN - 1096-9888

pISSN - 0887-6134

DOI - 10.1002/bms.1200180931

Subject(s) - mass spectrometry , ultraviolet , atmospheric pressure laser ionization , ionization , chemistry , laser , desorption , surface enhanced laser desorption/ionization , chromatography , analytical chemistry (journal) , sample preparation in mass spectrometry , materials science , ion , optoelectronics , electrospray ionization , electron ionization , optics , physics , adsorption , organic chemistry

Proteins with molecular weights between 14 000 and 175 000 daltons were investigated by matrix‐assisted laser desorption/ionization mass spectrometry. High abundance molecular ions were obtained with absolute sample amounts ranging from 5 pmol down to 50 fmol, allowing molecular weight determination with an accuracy between 0.1 and 0.2%. The amount of sample needed for an analysis is determined by the preparation rather than by the mass analysis step; the majority of the sample can be regained after analysis.

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