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Fast atom bombardment combined with tandem mass spectrometry for analysis of a complex mixture of lipo‐oligopeptides from Nocardia rubra cell wallskeleton
Author(s) -
Fujioka Mamoru,
Sentoh Fumiko,
Koda Shigetaka,
Morimoto Yukiyoshi
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200180920
Subject(s) - fast atom bombardment , tandem mass spectrometry , chemistry , mass spectrometry , collision induced dissociation , tandem , ion , dissociation (chemistry) , oligopeptide , analytical chemistry (journal) , chromatography , peptide , materials science , organic chemistry , biochemistry , composite material
Experimental variables in fast atom bombardment combined with tandem mass spectrometry based on low‐energy collision‐induced dissociation have been systematically invetigated in order to optimize parameters for analysis of synthetic lipo‐oligopeptides. The parameters studied were matrix, ion polarity, collision gas, collision gas pressure and collision energy. The optimal parameters recommended in this study have been applied to the N‐terminal amino acid seuquence determination of lipopeptide obtained from Nocardia rubra cell wall skeleton. Daughter and parent ion experiments with negative ion fast atom bombardment combined with tandem mass spectrometry have proven to be very useful for structure elucidation of a complex mixture of lipo‐oligopeptides.