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Gas chromatographic/mass spectrometric determination of alicyclic primary hydroxylamines in metabolic studies in vitro
Author(s) -
Kurebayashi Hideo,
Sato Michio,
Tanaka Akira
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200180913
Subject(s) - alicyclic compound , chemistry , hydroxylamine , chromatography , mass spectrum , mass spectrometry , gas chromatography , primary (astronomy) , primary metabolite , gas chromatography–mass spectrometry , organic chemistry , metabolite , biochemistry , physics , astronomy
The combination of gas chromatography and mass spectrometry (GC/MS) is effective for separation and identification of the hydroxylamine metabolites of alicyclic primary amines after acetylation. These products give mass spectra contianing diagnostic fragment ions which are of great value for identification of metabolites. The mass spectra of diacetyl alicyclic primary hydroxylamines gave prominent characteristic peaks at m/z (M – 42), (M – 42 – 42), (M – 101), 118 (AcNOAc) and 76 (AcNOH). GC/MS analysis of the incubation extracts has shown that the N ‐hydroxylamines are the major metabolites of alicyclic primary amines in rabbit liver microsomes.