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Structure analysis of modified oligodeoxyribonucleotides by negative ion fast atom bombardment mass spectrometry
Author(s) -
Iden Charles R.,
Rieger Robert A.
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200180818
Subject(s) - fast atom bombardment , mass spectrometry , ion , sequence (biology) , chemistry , mass spectrum , atom (system on chip) , nucleotide , analytical chemistry (journal) , chromatography , organic chemistry , biochemistry , computer science , gene , embedded system
Negative ion fast atom bombardment mass spectrometry is used to verify the sequence of oligodeoxyribonucleotides containing a single modified nucleotide. Both the position in the sequence and the molecular weight of the modified nucleotide can be determined from the 3′ and 5′‐phosphate sequence ions which are prominent in the mass spectrum.