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Determination of pipecolic acid in serum or plasma by solid‐phase extraction and isotope dilution mass spectrometry
Author(s) -
Van Bocxlaer J. F.,
Verhaeghe B. J.,
Wauters A. E.,
De Marez W. D.,
Thienpont L. M.,
De Leenheer A. P.
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200180810
Subject(s) - derivatization , chemistry , chromatography , isotope dilution , pipecolic acid , mass spectrometry , reagent , solid phase extraction , analyte , gas chromatography , chemical ionization , elution , extraction (chemistry) , gas chromatography–mass spectrometry , resolution (logic) , ion , ionization , organic chemistry , biochemistry , amino acid , artificial intelligence , computer science
The determination of pipecolic acid in serum or plasma by positive chemical ionization gas chromatography/mass spectrometry is assessed. This quantitative method involves stable isotope dilution and cation‐exchange solid‐phase extraction. Several derivatives of pipecolic acid and its octadeuterated analogue were investigated for their mass spectrometric characteristics. The beptafluorobutyric methyl ester derivatives afford optimal resolution on gas chromatography of biological extracts. Moreover, the derivatizing reagent (methanolic HCl) allows a combined elution and derivatization. Selected ion monitoring is performed on the [M + H] + ions of both analyte and internal standard, at m/z 340 and 348, respectively. Serum or plasma samples from healthy subjects and patients suspected of peroxisomal diseases have been examined.