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Studies on anabolic steroids II —gas chromatographic/mass spectrometric characterization of oxandrolone urinary metabolites in man
Author(s) -
Massé Robert,
Bi Honggang,
Ayotte Christiane,
Dugal Robert
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200180612
Subject(s) - oxandrolone , anabolism , chromatography , chemistry , gas chromatography , anabolic steroid , biochemistry , growth hormone , hormone
The metabolism of 17α‐methyl‐17β‐hydroxy‐2‐oxa‐5α‐androstan‐3‐one (oxandrolone) in man has been investigated by gas chromatography/mass spectrometry. After oral administration of a 10 mg dose to man, five metabolites were detected in the free fraction of the urinary samples. Oxandrolone, the major compound excreted in urine, was detected within 72 h after administration. During this period 35.8 and 8.4% of the administered dose was excreted as unchanged oxandrolone and 17‐epioxandrolone, respectively. In addition, minute amounts of 16α‐ and 16β‐ hydroxyoxandrolone and a δ‐hydroxy acid resulting from the hydrolysis of the lactone group of oxandrolone were detected in the urine samples 8–60 h after administration. Furthermore, the susceptibility of oxandrolone to hydrolysis was investigated under several pH conditions. Extraction and fractionation of steroidal metabolites was achieved by using C 18 and silica Sep Pak™ chromatography. The mass spectra of the metabolites are presented and major fragmentation pathways discussed.