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Factors affecting the fragmentation of peptides in fast atom bombardment mass spectrometry
Author(s) -
Naylor Stephen,
Moneti Gloriano
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200180608
Subject(s) - fragmentation (computing) , fast atom bombardment , chemistry , mass spectrometry , ion , derivatization , mass spectrum , matrix (chemical analysis) , matrix isolation , abundance (ecology) , atom (system on chip) , analytical chemistry (journal) , chromatography , molecule , organic chemistry , computer science , operating system , fishery , biology , embedded system
Abstract Positive ion fast atom bombardment mass spectra of a series of biologically active peptides were obtained in order to ascertain conditions that contribute to an increase in fragment ion abundance of such peptides. The effect of derivatization, hydrophobic character and the presence of acid in the matrix on fragmentation of peptides were all investigated. It is shown that a substantial increase in fragment signal ion abundance is observed when mineral acid is present in the matrix, and is in accord with the gas‐phase collision model proposed by Kebarle.