Premium
Identification of in vitro metabolites of 3‐ethyl‐2,6‐dimethyl‐4 H ‐pyrido(1,2 a )pyrimidin‐4‐one
Author(s) -
Jemnitz Katalin,
Dénes Géza,
Tamás József,
GácsBaitz Eszter
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200180505
Subject(s) - mass spectrometry , chemistry , electron ionization , mass spectrum , chromatography , ion , in vitro , thin layer chromatography , metastability , spectroscopy , microsome , ionization , organic chemistry , biochemistry , physics , quantum mechanics
(2‐ 14 C)3‐Ethyl‐2,6‐dimethyl‐4 H ‐pyrido(1,2 a )pyrimidin‐4‐one is metabolized in vitro by liver microsomes to at least 12 metabolites. The metabolites were isolated and purified by thin‐layer chromatography and high‐performance liquid chromatography and identified by mass spectrometry and nuclear magnetic resonance spectrosopy. The mostly mono‐ and dihydroxylated isomers were distinguished on the bases of their electron ionization mass spectra and the metastable daughter ion spectra of selected ions.