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Mass spectral investigations on trichothecene mycotoxins. VIII—thermospray ionization and collisionally activated dissociation of macrocyclic trichothecenes
Author(s) -
Krishnamurthy Thaiya,
Beck Debra J.,
Isensee Robert K.
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200180503
Subject(s) - chemistry , thermospray , dissociation (chemistry) , adduct , fragmentation (computing) , xanthene , chemical ionization , mass spectrometry , ion , mass spectrum , ionization , photochemistry , stereochemistry , tandem mass spectrometry , chromatography , organic chemistry , computer science , selected reaction monitoring , operating system
Several structurally similar, biologically active macrocyclic trichothecenes were ionized under thermospray conditions followed by collisionally activated dissociation of the ammonium adducts. Most of the observed daughter ions were formed by bond cleavage at the exocyclic ester bridges. Compounds with similar ester bridges formed several common daughter ions and underwent similar neutral losses. Fragmentation pathways proposed for the dissociation of the adducts were confirmed from the corresponding neutral loss spectra. Simple experiments designed for the sequential monitoring of the characteristic daughter ions were used for the rapid, direct and accurate analysis of macrocyclic trichothecenes in real samples. The primary drawback of the method is its inability to distinguish between isomers.