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The differentiation of methyl guanosine isomers by laser ionization fourier transform mass spectrometry
Author(s) -
Hettich R. L.
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200180410
Subject(s) - mass spectrometry , fourier transform , ionization , chemistry , analytical chemistry (journal) , guanosine , physics , chromatography , organic chemistry , ion , biochemistry , quantum mechanics
Abstract Laser ionization of guanosines containing methyl substitutions in the 1‐, N 2 ‐, 3′‐ O ‐, O 6 ‐ and 7‐ positions generated two characteristic negative ions: loss of hydrogen to generate [M – H] − and elimination of the sugar ring to form the nucleic base ion. The ions generated by elimination of the sugar ring provided the information necessary to determine whether the methyl group was on the nucleic base or sugar ring. Fourier transform mass spectrometry was used to isolate and collisionally dissociate selected negative ions from these nucleosides. The collisional dissociation spectra indicated daughter ions which were sufficient to differentiate all the isomers with methyl substitution of the nucleic bases. In addition, accurate mass measurement an dsequential collisional dissociation experiments were employed to investigate fragmentation mechanisms.