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Indole derivatization procedures for electron capture negative chemical ionization mass spectrometry: Identification of 1‐methyl‐1,2,3,4‐tetrahydro‐β‐carboline in rat brain and lung
Author(s) -
Bosin Talmage R.,
Faull Kym F.
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200180407
Subject(s) - derivatization , indole test , mass spectrometry , chemistry , electron ionization , chemical ionization , chromatography , electron capture , ionization , gas chromatography–mass spectrometry , mass spectrum , organic chemistry , ion
Procedures have been developed for the isolation of pharmacologically active indole compounds from biological samples and for the introduction of electron‐capturing groups, pentafluorobenzyl and trifluoroacetyl, onto the indole nitrogen atom. The resulting derivatives have good gas chromatographic properties and strong electron affinities which make them highly suitable for detection capture negative chemical ionization mass spectrometry. These procedures were used to identify 1‐methyl‐1,2,3,4‐tetrahydro‐β‐carboline as a component of rat brain and lung.