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Fast atom bombardment of metal–pyochelin complexes: Metastable analysis at constant B/E of zinc–pyochelin
Author(s) -
Beier Ross C.,
Stipanovic Robert D.
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200180307
Subject(s) - chemistry , ferric , ferrous , zinc , fast atom bombardment , acetic acid , inorganic chemistry , chloride , ion , organic chemistry
Fast atom bombardment (FAB) mass spectrometry was used to characterize the pyochelin complexes of iron and zinc. Ferripyochelin readily forms the [M + H] + and [2M + H] + ions in a glycerol matrix. The molecular ion was 2.5 fold more abundant when acetic acid was present. Iron was shown to be present in the iron‐pyochelin complex as the ferric ion by wet chemical methods. Differentiation between ferric and ferrous ions by FAB was not successful owing to the FAB reducing environment. Ferric ions were reduced by FAB to ferrous ions in the following model salts—ferric chloride, ferric nitrate and ferric sulfate—when dissolved in either glycerol, thioglycerol or the magic bullet matrix. Ferric and ferrous chloride salts gave virtually identical spectra with a glycerol/acetic acid matrix. Zinc‐pyochelin readily forms the [M + H] + and [2M + H] + ions, but only when acetic acid was present in the glycerol matrix. The FAB mass spectrum of zinc‐pyochelin exhibited various fragmentations which can be used in structural analysis. Linked‐scan at constant B/E and accurate mass data were used to characterize the fragmentations of the zinc‐pyochelin complex. Metastable analysis allowed the fragmentation pathway of zinc‐pyochelin to be determined.