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Peptide sequencing by partial methanolysis and fast atom bombardment mass spectrometry
Author(s) -
Foti Salvatore,
Saletti Rosaria
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200180304
Subject(s) - fast atom bombardment , mass spectrometry , peptide , sequence (biology) , chemistry , methanol , mass spectrum , peptide sequence , atom (system on chip) , amino acid , composition (language) , chromatography , spectral line , organic chemistry , biochemistry , physics , computer science , linguistics , philosophy , astronomy , gene , embedded system
The possibility of obtaining sequence information on peptides by partial methanolysis with 5 N HCl in dry methanol and subsequent fast atom bombardment (FAB) of the resulting mixture was investigated. This procedure was tested using four peptides of different size and amino acid composition. The results obtained demonstrate that this approach is effective in producing FAB spectra containing more sequence information than the spectra of the untreated peptides. For the compounds investigated the spectra contain enough information to unequivocally reassemble the original sequence.