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Suppression effects in peptide mapping by plasma desorption mass spectrometry
Author(s) -
Nielsen Per F.,
Roepstorff Peter
Publication year - 1989
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200180208
Subject(s) - mass spectrometry , chemistry , fast atom bombardment , desorption , static secondary ion mass spectrometry , peptide , ion , protein mass spectrometry , analytical chemistry (journal) , chromatography , sample preparation in mass spectrometry , tandem mass spectrometry , secondary ion mass spectrometry , electrospray ionization , biochemistry , adsorption , organic chemistry
Abstract Suppression effects observed in plasma desorption (PD) mass spectrometry have been studied and compared to those found in fast atom bombardment mass spectrometry. A basic difference in the mechanism of suppression in the two techniques is demonstrated. In positive ion mode PD mass spectrometry, peptides carrying net positive charges are preferentially detected when analysed together with peptides carrying net negative charges, and in negative ion mode PD mass spectrometry, the situation is generally reversed. Based on this complementarity, PD mapping carried out by enzymatic digestion of a nitrocellulose‐bound sample can in many cases yield almost complete coverage of smaller proteins.