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A gas chromatographic mass spectrometric method for the analysis of trifluoroacetic acid: Application to the metabolism of halothane by in vitro preparations
Author(s) -
Gruenke Larry D.,
Waskell Lucy A.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200170610
Subject(s) - trifluoroacetic acid , chromatography , chemistry , gas chromatography–mass spectrometry , halothane , sulfuric acid , gas chromatography , mass spectrometry , contamination , selected ion monitoring , organic chemistry , ecology , biology
A selected ion monitoring gas chromatographic mass spectrometric assay for trifluoroacetic acid was developed for the study of the metabolism of the volatile anesthetic agent halothane by in vitro preparations. The assay uses a headspace sampling technique after formation of methyl esters with dimethyl sulfate and sulfuric acid. Pentafluoropropionic acid proved to be a suitable internal standard, although care is required in the preparation of the calibration standards so that they reflect the composition and treatment of the samples. Contamination of the samples, possibly with trifluoroacetic acid itself, was found to be the primary factor in limiting the sensitivity of the assay for the metabolism of halothane. Generally, trifluoroacetic acid could be determined at levels as low as 1 μM in 50–100 μl of incubate.