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Femtomole analysis of diclofenac in human plasma by gas chromatography/negative ion chemical ionization mass spectrometry using ( 18 O 2 ) diclofenac as internal standard
Author(s) -
Leis H. J.,
Gleispach H.,
Nitsche V.,
Malle E.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200170604
Subject(s) - chemistry , chromatography , mass spectrometry , diclofenac , isotope dilution , derivatization , gas chromatography , gas chromatography–mass spectrometry , chemical ionization , ionization , ion , organic chemistry , biochemistry
A stable isotope dilution gas chromatographic/negative ion chemical ionization mass spectrometric assay for diclofenac in human plasma is described. The preparation of ( 18 O 2 )diclofenac and its use as an internal standard for quantitative gas chromatography/mass spectrometry is shown. A sample processing and derivatization sequence with product recovery of 84.7% was found. The method presented permits quantitative measurement of diclofenac in human plasma at the lower femtomole level. Plasma levels of diclofenac after administration of diclofenac gel were estimated.