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Optimization of sample preparation for plasma desorption mass spectrometry of peptides and proteins using a nitrocellulose matrix
Author(s) -
Nielsen Per F.,
Klarskov Klaus,
Højrup Peter,
Roepstorff Peter
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200170503
Subject(s) - nitrocellulose , sample preparation , matrix (chemical analysis) , mass spectrometry , chromatography , analytical chemistry (journal) , chemistry , desorption , sample (material) , trypsin , membrane , adsorption , enzyme , biochemistry , organic chemistry
Molecular ion yields and spectrum quality in plasma desorption mass spectrometry of peptides and proteins have been studied as a function of the method of sample of preparation. Parameters like matrix preparation, pH of the sample solution, method of applying the sample on the matrix and sample quantity have been studied. The best matrix was found to be nitrocellulose prepared by electrospraying. Various techniques for applying the sample result in different sensitivity. The highest sensitivity is obtained using the spin‐drying technique, which results in high‐quality spectra with 1–2 pmol of trypsin (mol. wt 23,463). The results obtained using different sample preparation techniques are discussed and directions for an optimal procedure are given.