Premium
Femtomole analysis of diclofenac in human plasma by gas chromatography/negative ion chemical ionization/mass spectrometry using ( 18 O 2 )diclofenac as internal standard
Author(s) -
Leist H. J.,
Gleispach H.,
Malle E.,
Nitsche V.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200170414
Subject(s) - chemistry , mass spectrometry , chromatography , isotope dilution , diclofenac , derivatization , gas chromatography , chemical ionization , gas chromatography–mass spectrometry , electron ionization , ionization , ion , organic chemistry , biochemistry
Abstract A stable isotope dilution gas chromatography/negative ion chemical ionization/mass spectrometry assay for diclofenac in human plasma is described. The preparation of ( 18 O 2 )diclofenac and its use as an internal standard for quantitative gas chromatography/mass spectrometry is shown. A sample processing and derivatization sequence with product recovery of 84.7% was found. The method presented permits quantitative measurement of diclofenac in human plasma at the lower femtomole level. Plasma levels of diclofenac after administration of diclofenac gel were estimated.