Premium
Methane chemical ionization mass spectrometry of the pentafluorobenzyl derivatives of abscisic acid, its metabolites and other plant growth regulators
Author(s) -
Netting A. G.,
Milborrow B. V.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200170409
Subject(s) - ammonium hydroxide , chemical ionization , chemistry , derivatization , mass spectrometry , methane , hydroxide , gibberellic acid , chromatography , ionization , ion , organic chemistry , biology , botany , germination
Tetraethyl ammonium hydroxide has been used as a catalyst for the pentafluorobenzylation of abscissic acid and its metabolites, as well as zeatin, gibberellic acid and indole acetic acid. These derivatives, acetylated where necessary, were subjected to methane negative ion chemical ionization mass spectrometry. The spectra were quite simple and are therefore ideally suited for selected ion monitoring at high sensitivity with heavy isotope labelled internal standards. The sensitivity for pentafluorobenzyl abscisate was 20 fg with a signal to noise ratio of greater than 10:1. The other compounds were within an order of magnitude of this. It is thus feasible, at least in principle, to quantify components from all four groups of plant growth regulators from the same tissue.