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Thermospray mass spectrometric analysis of phenazines
Author(s) -
Watson David,
Taylor Graham W.,
Wilson Robert,
Cole Peter J.,
Rowe Colin
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200170404
Subject(s) - thermospray , chemistry , phenazine , mass spectrum , mass spectrometry , chromatography , derivatization , fragmentation (computing) , electron ionization , gas chromatography , ion , organic chemistry , tandem mass spectrometry , biology , selected reaction monitoring , ecology , ionization
Positive and negative thermospray mass spectra have been recorded for a number of phenazines at the submicrogram level. The spectra are dominated by molecular ion species ([M + H] + , [M – H] − ) with minimal fragmentation. The gas chromatographic/electron impact mass spectrometric behaviour of the phenazines was also investigated. A number of phenazines were not amenable to gas chromatographic/mass spectrometric analysis without derivatization; in contrast, thermospray mass spectrometric data were obtained for each phenazine. Thermospray liquid chromatography/mass spectrometry has been applied to identify phenazine‐1‐carboxylic acid and 2‐hydroxyphenazine in culture filtrates of Pseudomonas aureofaciens and 1‐amidophenazine from Pseudomonas aeruginosa .