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Quantification of leukotriene B 4 in synovial fluid by gas chromatography/tandem mass spectrometry
Author(s) -
Dawson M.,
McGee C. M.,
Brooks P. M.,
Vine J. H.,
Watson T. R.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200170309
Subject(s) - leukotriene b4 , chemistry , chromatography , synovial fluid , mass spectrometry , gas chromatography , gas chromatography/tandem mass spectrometry , tandem mass spectrometry , detection limit , chemical ionization , gas chromatography–mass spectrometry , ion , ionization , medicine , organic chemistry , immunology , inflammation , alternative medicine , pathology , osteoarthritis
A procedure for the quantification of leukotriene B 4 (LTB 4 ) in synovial fluid has been developed using gas chromatography/tandem mass spectrometry based on selected reaction monitoring of the elimination of t ‐butyldimethylsilanol from the ions of m/z 431 and 438 in the negative ion chemical ionization mass spectra of the di‐ t ‐butyldimethylsilyl/pentafluorobenzyl derivatives of derivatives of leukotriene B 4 and the internal standard ( 2 H 8 )leukotriene B 4 . The detection limit (∼10 pg ml −1 ) is sufficiently low to permit determination of LTB 4 concentration in the synovial fluid of patients with various arthropathies. Single‐stage mass spectrometry was found not to be selective enough to permit quantification of LTB 4 in synovial fluid.