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The determination of the amino acid sequence in the fast atom bombardment mass spectra of dipeptides
Author(s) -
Kulik Willem,
Heerma Wigger
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200170305
Subject(s) - fragmentation (computing) , ion , mass spectrum , protonation , chemistry , fast atom bombardment , metastability , spectral line , amino acid , atom (system on chip) , mass spectrometry , physics , biology , biochemistry , chromatography , organic chemistry , ecology , astronomy , computer science , embedded system
The positive ion fast atom bombardment mass spectra of dipeptides all exhibit the MH + ion as the most abundant one. Direct identification of the N‐terminal amino acid in the mass spectrum is not possible because of the absence of its acylium ion. Fortunately the identification of the C‐terminius is often possible because ofthe occurrence of the protonated C‐terminal amino acid as a result of MH + ion fragmentation. In all cases, including those where the abundance of this C‐terminal ion is too low for identification, the metastable ion spectrum of the MH + ion provides sufficient information for an unequivocal sequence determination.