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Negative ion fast atom bombardment mass spectrometry of oligodeoxynucleotide carbamate analogs
Author(s) -
Griffin D.,
Laramée J.,
Deinzer M.,
Stirchak E.,
Weller D.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200170206
Subject(s) - fast atom bombardment , phosphodiester bond , chemistry , mass spectrometry , carbamate , oligonucleotide , cleavage (geology) , ion , sequence (biology) , stereochemistry , tandem mass spectrometry , dna , chromatography , organic chemistry , biochemistry , rna , gene , fracture (geology) , geotechnical engineering , engineering
Oligonucleotide analogs, in which carbamate rather than phosphodiester linkages form the backbone, have been analyzed by negative ion fast atom bombardment mass spectrometry. These oligodeoxynucleotides, dimers (982.4 daltons) to 11‐mers (4356.5 daltons), show intense [M – H] − ions and some degree of cleavage of the sugarcarbamate backbone structure in repeating fashion on both sides of the carbonyl groups. Sequencing of the shorter chain oligonucleotide analogs, up to six bases long, is demonstrated by complete 3′ and 5′ terminal sequence fragment ions. Longer chain oligomers show partial sequencing information.