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Phase‐system switching: A new approach in liquid chromatography/mass spectrometry coupling
Author(s) -
Verheij E. R.,
Reeuwijk H. J. E. M.,
Niessen W. M. A.,
Tjaden U. R.,
Van der Greef J.,
Verheij E. R.,
Lavos G. F.,
Van der Greef J.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200160177
Subject(s) - mass spectrometry , chemistry , chromatography , phase (matter) , analytical chemistry (journal) , volumetric flow rate , aqueous solution , coupling (piping) , materials science , physics , organic chemistry , quantum mechanics , metallurgy
A new approach is presented to overcome the problems imposed by the use of non‐volatile mobile phase constituents, such as buffers, in liquid chromatographic/mass spectrometric applications. The approach, called phase‐system switching (PSS), is based on the use of valve switching techniques. The peak of interest is heart‐cut from the effluent of the analytical column and trapped on a short trapping column, the buffer constituents are washed off, and subsequently the compound of interest is desorbed in a solvent favourable for the liquid chromatograph/mass spectrometer interface. The potential of the phase‐system switching technique is explored in the liquid chromatographic/mass spectrometric analysis of the anti‐cancer drug mitomycin C, which is chromatographed with an aqueous phosphate buffer as the mobile phase on a reversed‐phase column. In addition to the phase‐system switching itself, the PSS technique shows the interesting possibilities of peak compression and changes in flow rate.