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Analysis of fatty acids by negative ion gas chromatography/tandem mass spectrometry: Structural correlations between α‐mycolic acid chains and δ‐5‐monounsaturated fatty acids from Mycobacterium phlei
Author(s) -
Couderc F.,
Aurelle H.,
Promé D.,
Savagnac A.,
Promé J. C.
Publication year - 1988
Publication title -
biomedical and environmental mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 0887-6134
DOI - 10.1002/bms.1200160161
Subject(s) - chemistry , mass spectrometry , chromatography , dissociation (chemistry) , chemical ionization , fatty acid , gas chromatography , tandem mass spectrometry , electron ionization , carboxylate , mass spectrum , mycobacterium phlei , ion , ionization , stereochemistry , organic chemistry , bacteria , mycobacterium , biology , genetics
The analysis of a complex mixture of monounsaturated fatty acids from Mycobacterium phlei is achieved by capillary gas chromatographic separation of their pentafluorobenzyl esters, formation of gas‐phase carboxylate anions by electron capture ionization and decomposition of these anions by collision activation. This method allows the structural determination of fatty acid isomers by examination of their collisionally activated dissociation mass‐analysed ion kinetic energy spectra. A good correlation is observed between the structure of the unsaturated fatty acids ranging from C24 to C27 and the methyl terminal part of the main chain of diunsaturated mycolic acids.